Protocols for sample preparation and compound-specific stable-isotope analyses (δ2H, δ13C) of fatty acids in biological and environmental samples
Pilecky, M., Wassenaar, L. I., Taipale, S., & Kainz, M. J. (2023). Protocols for sample preparation and compound-specific stable-isotope analyses (δ2H, δ13C) of fatty acids in biological and environmental samples. MethodsX, 11, Article 102283. https://doi.org/10.1016/j.mex.2023.102283
Julkaistu sarjassa
MethodsXPäivämäärä
2023Tekijänoikeudet
© 2023 The Author(s). Published by Elsevier B.V.
Compound-specific stable-isotope analysis (CSIA) of fatty acids is a powerful tool to better understand the trophic transfer of fatty acids and their biochemical fate in and across ecosystems, including tracing animal migration and understanding physiological processes. The non-exchangeable nature of C–H bonds in acyl chains, hydrogen (𝛿2H) and carbon (𝛿13C) stable-isotope values of fatty acids (FA) provide independent information about the origins of fatty acids. Several technical obstacles must be overcome to ensure accurate and reproducible measurements of FA-CSIA can be made. This protocol describes the sample preparation process for successful stable-isotope analyses of fatty acids obtained from environmental and biological samples. Numerous techniques for the preanalytical processing of fatty acid samples are available, and these often have minimal impact on 𝛿 values. Here, we provide an in-depth guide detailing our well-established laboratory protocols, ranging from the initial sample preparation, lipid extraction, and transmethylation to the instrumental arrangement, data collection, and analysis.
• Protocol from obtaining a sample to standardized fatty acid specific 𝛿2H and 𝛿13C values.
• Separate GC analysis procedures for C and H are recommended for optimal performance.
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Elsevier BVISSN Hae Julkaisufoorumista
2215-0161Asiasanat
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https://converis.jyu.fi/converis/portal/detail/Publication/184012551
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This work has been supported by the Austrian Science Fund (P-36250) to LIW.Lisenssi
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