Arginine Methyltransferase PRMT7 Deregulates Expression of RUNX1 Target Genes in T-Cell Acute Lymphoblastic Leukemia
Oksa, Laura; Mäkinen, Artturi; Nikkilä, Atte; Hyvärinen, Noora; Laukkanen, Saara; Rokka, Anne; Haapaniemi, Pekka; Seki, Masafumi; Takita, Junko; Kauko, Otto; Heinäniemi, Merja; Lohi, Olli (2022)
Oksa, Laura
Mäkinen, Artturi
Nikkilä, Atte
Hyvärinen, Noora
Laukkanen, Saara
Rokka, Anne
Haapaniemi, Pekka
Seki, Masafumi
Takita, Junko
Kauko, Otto
Heinäniemi, Merja
Lohi, Olli
2022
2169
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi:tuni-202205094541
https://urn.fi/URN:NBN:fi:tuni-202205094541
Kuvaus
Peer reviewed
Tiivistelmä
T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive hematological malignancy with no well-established prognostic biomarkers. We examined the expression of protein arginine methyltransferases across hematological malignancies and discovered high levels of PRMT7 mRNA in T-ALL, particularly in the mature subtypes of T-ALL. The genetic deletion of PRMT7 by CRISPR-Cas9 reduced the colony formation of T-ALL cells and changed arginine monomethylation patterns in protein complexes associated with the RNA and DNA processing and the T-ALL pathogenesis. Among them was RUNX1, whose target gene expression was consequently deregulated. These results suggest that PRMT7 plays an active role in the pathogenesis of T-ALL.
Kokoelmat
- TUNICRIS-julkaisut [16944]