Murine cerebral organoids develop network of functional neurons and hippocampal brain region identity
Ciarpella, Francesca; Zamfir, Raluca Georgiana; Campanelli, Alessandra; Ren, Elisa; Pedrotti, Giulia; Bottani, Emanuela; Borioli, Andrea; Caron, Davide; Di Chio, Marzia; Dolci, Sissi; Ahtiainen, Annika; Malpeli, Giorgio; Malerba, Giovanni; Bardoni, Rita; Fumagalli, Guido; Hyttinen, Jari; Bifari, Francesco; Palazzolo, Gemma; Panuccio, Gabriella; Curia, Giulia; Decimo, Ilaria (2021-12-17)
Ciarpella, Francesca
Zamfir, Raluca Georgiana
Campanelli, Alessandra
Ren, Elisa
Pedrotti, Giulia
Bottani, Emanuela
Borioli, Andrea
Caron, Davide
Di Chio, Marzia
Dolci, Sissi
Ahtiainen, Annika
Malpeli, Giorgio
Malerba, Giovanni
Bardoni, Rita
Fumagalli, Guido
Hyttinen, Jari
Bifari, Francesco
Palazzolo, Gemma
Panuccio, Gabriella
Curia, Giulia
Decimo, Ilaria
17.12.2021
103438
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi:tuni-202112289545
https://urn.fi/URN:NBN:fi:tuni-202112289545
Kuvaus
Peer reviewed
Tiivistelmä
Brain organoids are in vitro three-dimensional (3D) self-organized neural structures, which can enable disease modeling and drug screening. However, their use for standardized large-scale drug screening studies is limited by their high batch-to-batch variability, long differentiation time (10–20 weeks), and high production costs. This is particularly relevant when brain organoids are obtained from human induced pluripotent stem cells (iPSCs). Here, we developed, for the first time, a highly standardized, reproducible, and fast (5 weeks) murine brain organoid model starting from embryonic neural stem cells. We obtained brain organoids, which progressively differentiated and self-organized into 3D networks of functional neurons with dorsal forebrain phenotype. Furthermore, by adding the morphogen WNT3a, we generated brain organoids with specific hippocampal region identity. Overall, our results showed the establishment of a fast, robust and reproducible murine 3D in vitro brain model that may represent a useful tool for high-throughput drug screening and disease modeling.
Kokoelmat
- TUNICRIS-julkaisut [16726]