Effect of inactivated nature‐derived microbial composition on mouse immune system
González-Rodriguez, Martin Ignacio; Nurminen, Noora; Kummola, Laura; Laitinen, Olli; Oikarinen, Sami; Parajuli, Anirudra; Salomaa, Tanja; Mäkelä, Iida; Roslund, Marja I.; Sinkkonen, Aki; Hyöty, Heikki; Junttila, Ilkka (2021-12-06)
González-Rodriguez, Martin Ignacio
Nurminen, Noora
Kummola, Laura
Laitinen, Olli
Oikarinen, Sami
Parajuli, Anirudra
Salomaa, Tanja
Mäkelä, Iida
Roslund, Marja I.
Sinkkonen, Aki
Hyöty, Heikki
Junttila, Ilkka
06.12.2021
Immunity, Inflammation And Disease
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi:tuni-202112109104
https://urn.fi/URN:NBN:fi:tuni-202112109104
Kuvaus
Peer reviewed
Tiivistelmä
Introduction: The hygiene hypothesis suggests that decrease in early life infections due to increased societal-level hygiene standards subjects one to allergic and autoimmune diseases. In this report, we have studied the effect of sterilized forest soil and plant-based material on mouse immune system and gut microbiome. Methods: Inbred C57Bl/6 mice maintained in normal sterile environment were subjected to autoclaved forest soil-derived powder in their bedding for 1 h a day for 3 weeks. Immune response was measured by immune cell flow cytometry, serum cytokine enzyme-linked immunoassay (ELISA) and quantitative polymerase chain reaction (qPCR) analysis. Furthermore, the mouse gut microbiome was analyzed by sequencing. Results: When compared to control mice, mice treated with soil-derived powder had decreased level of pro-inflammatory cytokines namely interleukin (IL)−17F and IL-21 in the serum. Furthermore, splenocytes from mice treated with soil-derived powder expressed less IL-1b, IL-5, IL-6, IL-13, and tumor necrosis factor (TNF) upon cell activation. Gut microbiome appeared to be stabilized by the treatment. Conclusions: These results provide insights on the effect of biodiversity on murine immune system in sterile environment. Subjecting mice to soil-based plant and microbe structures appears to elicit immune response that could be beneficial, for example, in type 2 inflammation-related diseases, that is, allergic diseases.
Kokoelmat
- TUNICRIS-julkaisut [16944]