Detection of Hypoxia-inducible mRNAs in the Plasma of Non- Small Cell Lung Cancer Patients
HYRSKYLUOTO, ALISE (2009)
HYRSKYLUOTO, ALISE
2009
Biokemia - Biochemistry
Lääketieteellinen tiedekunta - Faculty of Medicine
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Hyväksymispäivämäärä
2009-02-17
Julkaisun pysyvä osoite on
https://urn.fi/urn:nbn:fi:uta-1-19605
https://urn.fi/urn:nbn:fi:uta-1-19605
Tiivistelmä
Abstract
Background and aims: The aim of this study was to investigate the mRNA expression levels of hypoxia-inducible factor 1α and three hypoxia-inducible genes, CA9, CA12 and OPN, in NSCLC patients' plasma and to evaluate their potential as clinical tumor markers. Lung cancer is one of the most common malignancies in the world and the leading cause of cancer-related deaths. Plasma biomarkers have not yet been available as an effective clinical tool in screening or early diagnosis of lung cancer. The discovery of new potential tumor markers would have positive impact on the clinical outcome of lung cancer patients by helping to detect the disease in an early phase.
Methods: mRNA expression of HIF-1α, OPN, CA IX and CA XII was studied by quantitative real-time polymerase chain reaction (qRT-PCR). Two house-keeping genes, B2M and UBC, were used for normalization. mRNA levels were assessed in the 95 blood plasma samples of NSCLC patients and 24 blood plasma samples of healthy volunteers. The qRT-PCR results were statistically analysed.
Results: No significant CA IX and CA XII expression was found to be detectable in blood plasma samples. Reasonable signals were obtained for OPN and HIF-1α and two house-keeping genes, UBC and B2M. Elevated mRNA levels were observed in cancer patients' plasma. Statistically significant difference was found between UBC and OPN mRNA levels of healthy controls and NSCLC patients. The majority of correlations between mRNA levels and clinical parameters or blood biomarkers were not statistically significant. Neither did the survival analysis show any significant relationship between survival and mRNA levels.
Conclusions: Due to low levels of circulating RNA in plasma quantitative real-time polymerase chain reaction seems to be challenging for routine diagnostics. In this study, a statistically significant difference was found in UBC and OPN mRNA levels between the healthy controls and NSCLC patients. Therefore, it is possible that the plasma RNA levels have some diagnostic value, although the test specificities and sensitivities remained quite low compared to the requirements set for routine laboratory diagnostics.
Background and aims: The aim of this study was to investigate the mRNA expression levels of hypoxia-inducible factor 1α and three hypoxia-inducible genes, CA9, CA12 and OPN, in NSCLC patients' plasma and to evaluate their potential as clinical tumor markers. Lung cancer is one of the most common malignancies in the world and the leading cause of cancer-related deaths. Plasma biomarkers have not yet been available as an effective clinical tool in screening or early diagnosis of lung cancer. The discovery of new potential tumor markers would have positive impact on the clinical outcome of lung cancer patients by helping to detect the disease in an early phase.
Methods: mRNA expression of HIF-1α, OPN, CA IX and CA XII was studied by quantitative real-time polymerase chain reaction (qRT-PCR). Two house-keeping genes, B2M and UBC, were used for normalization. mRNA levels were assessed in the 95 blood plasma samples of NSCLC patients and 24 blood plasma samples of healthy volunteers. The qRT-PCR results were statistically analysed.
Results: No significant CA IX and CA XII expression was found to be detectable in blood plasma samples. Reasonable signals were obtained for OPN and HIF-1α and two house-keeping genes, UBC and B2M. Elevated mRNA levels were observed in cancer patients' plasma. Statistically significant difference was found between UBC and OPN mRNA levels of healthy controls and NSCLC patients. The majority of correlations between mRNA levels and clinical parameters or blood biomarkers were not statistically significant. Neither did the survival analysis show any significant relationship between survival and mRNA levels.
Conclusions: Due to low levels of circulating RNA in plasma quantitative real-time polymerase chain reaction seems to be challenging for routine diagnostics. In this study, a statistically significant difference was found in UBC and OPN mRNA levels between the healthy controls and NSCLC patients. Therefore, it is possible that the plasma RNA levels have some diagnostic value, although the test specificities and sensitivities remained quite low compared to the requirements set for routine laboratory diagnostics.