Scanning transmission soft X-ray spectromicroscopy of mouse kidney and liver
Mansikkala, Tuomas; Ohigashi, Takuji; Salo, Miia H.; Hiltunen, Anniina E.; Vuolteenaho, Reetta; Sipilä, Petra; Kuure, Satu; Huttula, Marko; Uusimaa, Johanna; Hinttala, Reetta; Miinalainen, Ilkka; Kangas, Salla; Patanen, Minna (2023-07-05)
Mansikkala, T., Ohigashi, T., Salo, M. H., Hiltunen, A. E., Vuolteenaho, R., Sipilä, P., Kuure, S., Huttula, M., Uusimaa, J., Hinttala, R., Miinalainen, I., Kangas, S., & Patanen, M. (2023). Scanning transmission soft X-ray spectromicroscopy of mouse kidney and liver. Journal of Electron Spectroscopy and Related Phenomena, 266, 147368. https://doi.org/10.1016/j.elspec.2023.147368
© 2023 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
https://creativecommons.org/licenses/by/4.0/
https://urn.fi/URN:NBN:fi-fe20230921134638
Tiivistelmä
Abstract
Scanning transmission X-ray microscopy (STXM) in the soft X-ray range is well-suited to study ultrastructural features of mammalian soft tissues. Especially at the carbon 1s edge, the imaging contrast varies drastically across the edge due to rapid changes in the X-ray absorption cross-section of functional groups present in the tissue samples enabling label-free soft X-ray spectromicroscopic studies. We present STXM spectromicroscopic imaging of mouse kidney and liver tissues. We especially concentrate on ultrastructural abnormalities in genetically modified Slc17a5 mice. STXM is a promising technique to study storage diseases without chemical alteration due to staining agents, but sample preparation poses a challenge.
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