Interaction of gelatin with polyenes modulates antifungal activity and biocompatibility of electrospun fiber mats
Lakshminarayanan, Rajamani; Sridhar, Radhakrishnan; Loh Xian, Jun; Nandhakumar, Murugantham; Barathi, Veluchamy Amutha; Kalaipriya, Madhaiyan; Kwan Jia, Lin; Liu Shou, Ping; Beuerman, Roger W; Ramakrishna, Seeram (2014)
Lakshminarayanan, Rajamani
Sridhar, Radhakrishnan
Loh Xian, Jun
Nandhakumar, Murugantham
Barathi, Veluchamy Amutha
Kalaipriya, Madhaiyan
Kwan Jia, Lin
Liu Shou, Ping
Beuerman, Roger W
Ramakrishna, Seeram
2014
International Journal of Nanomedicine 23 9
2439-2458
Lääketieteen yksikkö - School of Medicine
CC BY-NC-3.0
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi:uta-201611182623
https://urn.fi/URN:NBN:fi:uta-201611182623
Tiivistelmä
Topical application of antifungals does not have predictable or well-controlled release characteristics and requires reapplication to achieve therapeutic local concentration in a reasonable time period. In this article, the efficacy of five different US Food and Drug Administration-approved antifungal-loaded (amphotericin B, natamycin, terbinafine, fluconazole, and itraconazole) electrospun gelatin fiber mats were compared. Morphological studies show that incorporation of polyenes resulted in a two-fold increase in fiber diameter and the mats inhibit the growth of yeasts and filamentous fungal pathogens. Terbinafine-loaded mats were effective against three filamentous fungal species. Among the two azole antifungals compared, the itraconazole-loaded mat was potent against Aspergillus strains. However, activity loss was observed for fluconazole-loaded mats against all of the test organisms. The polyene-loaded mats displayed rapid candidacidal activities as well. Biophysical and rheological measurements indicate strong interactions between polyene antifungals and gelatin matrix. As a result, the polyenes stabilized the triple helical conformation of gelatin and the presence of gelatin decreased the hemolytic activity of polyenes. The polyene-loaded fiber mats were noncytotoxic to primary human corneal and sclera fibroblasts. The reduction of toxicity with complete retention of activity of the polyene antifungal-loaded gelatin fiber mats can provide new opportunities in the management of superficial skin infections.
Kokoelmat
- Artikkelit [6140]