Protection against live rotavirus challenge in mice induced by parenteral and mucosal delivery of VP6 subunit rotavirus vaccine
Lappalainen, Suvi; Pastor, Ana Ruth; Malm, Maria; López-Guerrero, Vanessa; Esquivel-Guadarrama, Fernando; Palomares, Laura A.; Vesikari, Timo; Blazevic, Vesna (2015)
Lappalainen, Suvi
Pastor, Ana Ruth
Malm, Maria
López-Guerrero, Vanessa
Esquivel-Guadarrama, Fernando
Palomares, Laura A.
Vesikari, Timo
Blazevic, Vesna
2015
Archives of Virology 160 8
2075–2078
Lääketieteen yksikkö - School of Medicine
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi:uta-201606282040
https://urn.fi/URN:NBN:fi:uta-201606282040
Tiivistelmä
Live oral rotavirus (RV) vaccines are part of
routine childhood immunization but are associated with
adverse effects, particularly intussusception. We have developed
a non-live combined RV – norovirus (NoV) vaccine
candidate consisting of human RV inner-capsid rVP6
protein and NoV virus-like particles. To determine the
effect of delivery route on induction of VP6-specific protective
immunity, BALB/c mice were administered a vaccine
containing RV rVP6 intramuscularly, intranasally or a
combination of both, and challenged with murine RV. At
least 65 % protection against RV shedding was observed
regardless of delivery route. The levels of post-challenge
serum VP6-specific IgA titers correlated with protection.
routine childhood immunization but are associated with
adverse effects, particularly intussusception. We have developed
a non-live combined RV – norovirus (NoV) vaccine
candidate consisting of human RV inner-capsid rVP6
protein and NoV virus-like particles. To determine the
effect of delivery route on induction of VP6-specific protective
immunity, BALB/c mice were administered a vaccine
containing RV rVP6 intramuscularly, intranasally or a
combination of both, and challenged with murine RV. At
least 65 % protection against RV shedding was observed
regardless of delivery route. The levels of post-challenge
serum VP6-specific IgA titers correlated with protection.
Kokoelmat
- Artikkelit [6140]