Cancer associated talin point mutations disorganise cell adhesion and migration
Azizi, Latifeh; Cowell, Alana R.; Mykuliak, Vasyl V.; Goult, Benjamin T.; Turkki, Paula; Hytönen, Vesa P. (2021)
Azizi, Latifeh
Cowell, Alana R.
Mykuliak, Vasyl V.
Goult, Benjamin T.
Turkki, Paula
Hytönen, Vesa P.
2021
347
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi:tuni-202104193112
https://urn.fi/URN:NBN:fi:tuni-202104193112
Kuvaus
Peer reviewed
Tiivistelmä
Talin-1 is a key component of the multiprotein adhesion complexes which mediate cell migration, adhesion and integrin signalling and has been linked to cancer in several studies. We analysed talin-1 mutations reported in the Catalogue of Somatic Mutations in Cancer database and developed a bioinformatics pipeline to predict the severity of each mutation. These predictions were then assessed using biochemistry and cell biology experiments. With this approach we were able to identify several talin-1 mutations affecting integrin activity, actin recruitment and Deleted in Liver Cancer 1 localization. We explored potential changes in talin-1 signalling responses by assessing impact on migration, invasion and proliferation. Altogether, this study describes a pipeline approach of experiments for crude characterization of talin-1 mutants in order to evaluate their functional effects and potential pathogenicity. Our findings suggest that cancer related point mutations in talin-1 can affect cell behaviour and so may contribute to cancer progression.
Kokoelmat
- TUNICRIS-julkaisut [16983]