A modular LabVIEW program for controlling multimodal microscope imaging platform
Yuan, Qi (2020)
Yuan, Qi
2020
Automaatiotekniikan DI-tutkinto-ohjelma - Degree Programme in Automation Engineering, MSc (Tech)
Tekniikan ja luonnontieteiden tiedekunta - Faculty of Engineering and Natural Sciences
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Hyväksymispäivämäärä
2020-05-22
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi:tuni-202004284176
https://urn.fi/URN:NBN:fi:tuni-202004284176
Tiivistelmä
Automating a multi-device imaging setup is often needed in the bioengineering field not only to enable effective image data acquisition but also to enhance the accuracy of information content. Multimodal imaging shows great potential in the growing field on biosciences, for example, in cell and tissue culture imaging.
This master thesis project aims to develop and build a modular, loose-coupled LabVIEW software that automates the multimodal microscopy imaging platform which integrates the optical projection tomography (OPT) and electrical impedance tomography (EIT). The integrated software must enable OPT and EIT imaging modes and most importantly it allows fast simultaneous data acquisition for OPT/EIT imaging mode within a full 360◦ rotation. The data content acquired from the integrated software should be of high quality and usable for image reconstruction. Meanwhile, the architecture of the software should be modularized and extensible for further development.
The integrated software was implemented with LabVIEW, and it is able to provide a user
interface with three different imaging modes: OPT, EIT and OPT/EIT. Producer-consumer and event-driven design patterns were utilized into the software architecture and Object-Oriented Programming (OOP) was adopted for the implementation of individual software components, which makes the integrated software modular and scalable. In addition, various tests were implemented to assess the integrated software in terms of time performance, the accuracy of imaging data and the feasibilities of simultaneous rotational EIT and OPT data acquisition with enhanced time-cost performance.
Through the tests, the integrated software demonstrated that it acquired the same quality data compared with data from original software regarding the precision of data content from three modes and it also enables synchronous imaging data acquisition with an excellent time-cost performance that shortens the whole imaging time significantly. Besides, it simplified the workflow of the imaging process regardless of whatever imaging mode is selected and also the configuration of the imaging setup as well. Additionally, the integrated software is modular that makes it possible to incorporate other functionalities. Therefore, the integrated software fulfills the requirements of the thesis.
In the future, if other new, innovative imaging methods are added into the LabVIEW software, the integrated software will provide more flexibility and the possibility to acquire different imaging data all at once.
This master thesis project aims to develop and build a modular, loose-coupled LabVIEW software that automates the multimodal microscopy imaging platform which integrates the optical projection tomography (OPT) and electrical impedance tomography (EIT). The integrated software must enable OPT and EIT imaging modes and most importantly it allows fast simultaneous data acquisition for OPT/EIT imaging mode within a full 360◦ rotation. The data content acquired from the integrated software should be of high quality and usable for image reconstruction. Meanwhile, the architecture of the software should be modularized and extensible for further development.
The integrated software was implemented with LabVIEW, and it is able to provide a user
interface with three different imaging modes: OPT, EIT and OPT/EIT. Producer-consumer and event-driven design patterns were utilized into the software architecture and Object-Oriented Programming (OOP) was adopted for the implementation of individual software components, which makes the integrated software modular and scalable. In addition, various tests were implemented to assess the integrated software in terms of time performance, the accuracy of imaging data and the feasibilities of simultaneous rotational EIT and OPT data acquisition with enhanced time-cost performance.
Through the tests, the integrated software demonstrated that it acquired the same quality data compared with data from original software regarding the precision of data content from three modes and it also enables synchronous imaging data acquisition with an excellent time-cost performance that shortens the whole imaging time significantly. Besides, it simplified the workflow of the imaging process regardless of whatever imaging mode is selected and also the configuration of the imaging setup as well. Additionally, the integrated software is modular that makes it possible to incorporate other functionalities. Therefore, the integrated software fulfills the requirements of the thesis.
In the future, if other new, innovative imaging methods are added into the LabVIEW software, the integrated software will provide more flexibility and the possibility to acquire different imaging data all at once.