Determination carbohydrates in pine bark sample by capillary electrophoresis
Le, Tran (2018)
Le, Tran
Centria-ammattikorkeakoulu
2018
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https://urn.fi/URN:NBN:fi:amk-2018122322828
https://urn.fi/URN:NBN:fi:amk-2018122322828
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The topic of the thesis focuses on the identification and quantification of carbohydrate in Pine Bark sample by Capillary Electrophoresis. The first objective of this thesis is research, establish and evaluate the key factors which influence the analysis process. Based on the main factors, the best analytical parameters are researched to produce high accuracy and efficiency result. The second main goal is determinate and quantify monosaccharides in the available extracted Pine Bark sample by the capillary electrophoresis equipment.
There are six monosaccharides that are predicted in Pine Bark samples: Glucose, Arabinose, Galactose, Mannose, Fructose and Xylose. Five sugars appeared in extracted bark sample with different amounts consists of Arabinose, Galactose, Fructose, Glucose and Xylose. Analytical procedures were performed by capillary electrophoresis with UV detector at wavelength 270 nm. The monosaccharides move through the electro osmotic flow (EOF) of the electrolyte solution which contains 130 mM NaOH 1M: 36 mM Na2HPO4 pH 12.65 (±0.02). In particular, the flow of EOF and the analyte are influenced by the voltage of 20 kV at 20 ° C. The migration time of all carbohydrates between 11 and 14 minutes with the RSDs is 2%. For quantification, the range of amount for calibration is 10 – 60 ppm. Pine Bark sample was extracted with hot water or ethanol, then sample was filtered and diluted 1:2 with de-ionized water before analysis by Capillary Electrophoresis. All the calculation of quantification was done automatically and described on the printed report.
There are six monosaccharides that are predicted in Pine Bark samples: Glucose, Arabinose, Galactose, Mannose, Fructose and Xylose. Five sugars appeared in extracted bark sample with different amounts consists of Arabinose, Galactose, Fructose, Glucose and Xylose. Analytical procedures were performed by capillary electrophoresis with UV detector at wavelength 270 nm. The monosaccharides move through the electro osmotic flow (EOF) of the electrolyte solution which contains 130 mM NaOH 1M: 36 mM Na2HPO4 pH 12.65 (±0.02). In particular, the flow of EOF and the analyte are influenced by the voltage of 20 kV at 20 ° C. The migration time of all carbohydrates between 11 and 14 minutes with the RSDs is 2%. For quantification, the range of amount for calibration is 10 – 60 ppm. Pine Bark sample was extracted with hot water or ethanol, then sample was filtered and diluted 1:2 with de-ionized water before analysis by Capillary Electrophoresis. All the calculation of quantification was done automatically and described on the printed report.