Application of vitrification-derived cryotechniques for long-term storage of poplar and aspen (Populus spp.) germplasm
Tsvetkov, Ivaylo; Benelli, Carla; Capuana, Maurizio; De Carlo, Anna; Lambardi, Maurizio (2009)
Tsvetkov, Ivaylo
Benelli, Carla
Capuana, Maurizio
De Carlo, Anna
Lambardi, Maurizio
Julkaisusarja
Agricultural and Food Science
Volyymi
18
Numero
2
Sivut
160-166
MTT Agrifood Research Finland The Scientific Agricultural Society of Finland
2009
Julkaisun pysyvä osoite on
http://urn.fi/URN:NBN:fi-fe2015090311188
http://urn.fi/URN:NBN:fi-fe2015090311188
Tiivistelmä
The application of three different vitrification-based freezing strategies for the cryostorage of white poplar (Populus alba L.) and hybrid aspen (P. tremula L. × P. tremuloides Michx.) have been assessed. The PVS2 vitrification protocol was successfully applied to two white poplar in vitro clones stored for more than 6 months in slow-growth conditions (4 °C, in darkness) and showing clear signs of explant etiolation and decay. After 60 min of PVS2 treatment, P. alba L. (cv. Villafranca) explants isolated from axillary buds demonstrated significantly better potential for post-freeze regrowth (64%) compared to those obtained from apical buds (17%). Similarly, a high level of survival (78%) of the frozen hybrid aspen shoot tips was recorded following the application of the same technique. Using the encapsulation-vitrification procedure, no toxic effects of the PVS2 treatment were noticed after 120 min exposure, however none of the cryopreserved (poplar and aspen) explants survived after 3 weeks. In contrast, the droplet-vitrification technique appeared to be very efficient in the cryopreservation of white poplar shoot tips, which increases the opportunities for wider application of this method in other woody species.